Anti-Galectin 3/LGALS3 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, FC, ELISA - P17931

Core Product Specifications and Parameters

Biological Background: Galectin-3 Function and Localization

• Galectin-3 (also known as MAC2, CBP35, LGALS3) is a galactose-specific lectin that binds IgE and mediates cellular processes such as adhesion and signaling.
• It functions as a pre-mRNA splicing factor in the nucleus and coordinates autophagy with TRIM16 in response to endomembrane damage.
• Galectin-3 is involved in acute inflammatory responses, including neutrophil activation, monocyte chemoattraction, and opsonization of apoptotic neutrophils.
• It localizes to the cytoplasm, nucleus, and is also secreted, reflecting its multifunctional roles in intracellular and extracellular compartments.
• Major tissue expression includes colonic epithelium and activated macrophages; also present in fetal membranes.
• Post‑translational modifications include acetylation, phosphorylation, and disulfide bond formation, which may regulate its activity and localization.
• Key biological processes associated with Galectin-3 are cell differentiation, innate immunity, mRNA processing, and mRNA splicing.

Experimental Guidance and Technical Tips

• For Western blot, consider using reducing conditions to account for potential disulfide bonds; the predicted molecular weight is ~26 kDa.
• In immunofluorescence (IF/ICC), permeabilization is recommended to detect nuclear and cytoplasmic pools; secreted forms may require specific fixation protocols.
• For immunoprecipitation, the antibody may be effective with native protein conformations; validate under your experimental conditions.
• For flow cytometry, note that Galectin-3 is primarily intracellular and secreted; surface staining may require cell activation or permeabilization.
• Cross‑reactivity with mouse allows the antibody to be used in mouse models; always include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement, offering this anti-Galectin-3 monoclonal antibody for studies in inflammation, cancer biomarkers, and lectin biology. We supply validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets—supporting your diagnostic development from concept to clinic. Let CamelBio be your trusted partner in accelerating assay readiness and reducing supply chain complexity.