Anti-Galectin 3/LGALS3 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P17931

Core Product Specifications and Parameters

Biological Background: Galectin-3 Function and Localization

• Galectin-3 is a galactose-specific lectin encoded by the LGALS3 gene, also known as MAC2, CBP35, and L31.
• It binds IgE and mediates endothelial cell migration via integrin alpha-3/beta-1, and together with DMBT1, is required for terminal differentiation of columnar epithelial cells during early embryogenesis.
• In the nucleus, galectin-3 acts as a pre-mRNA splicing factor, participating in mRNA processing.
• It is involved in acute inflammatory responses, including neutrophil activation and adhesion, chemoattraction of monocytes/macrophages, opsonization of apoptotic neutrophils, and mast cell activation.
• Galectin-3 coordinates recognition of membrane damage with autophagy by interacting with TRIM16 to recruit ATG16L1 and BECN1 in response to damaged endomembranes.
• Subcellularly, it is found in the cytoplasm, nucleus, and secreted into the extracellular space.
• It is highly expressed in colonic epithelium, activated macrophages, and fetal membranes.
• Post-translational modifications include acetylation and phosphorylation; the protein contains a carbohydrate-recognition domain (CRD) and a repeat structure.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to the full-length human galectin-3 (aa 1–250); this antibody may recognize both native and denatured forms under reducing and non-reducing conditions.
• For Western blot (WB), a band of approximately 26 kDa is expected; adjust loading controls accordingly.
• In immunoprecipitation (IP), validate the antibody with relevant cell lysates or tissue homogenates expressing galectin-3.
• For immunofluorescence/immunocytochemistry (IF/ICC), consider fixation and permeabilization methods appropriate for the nuclear/cytoplasmic localization of galectin-3.
• Cross-reactivity with mouse and rat is reported; verify performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive IVD raw material solutions, including this rabbit monoclonal antibody against galectin-3, suitable for studying inflammation, cancer, and autophagy. As a trusted sourcing partner, CamelBio offers optimized antibody pairs, bulk reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic.