Anti-Galectin-1 Polyclonal Antibody for WB, IF/ICC, ELISA - P09382

Core Product Specifications and Parameters

Biological Background: Galectin-1 Function and Localization

• Galectin-1 (LGALS1), also known as 14 kDa laminin-binding protein (HLBP14) or S-Lac lectin 1, is a beta-galactoside-binding lectin that belongs to the galectin family.
• It plays critical roles in regulating apoptosis, cell proliferation, and cell differentiation by binding to beta-galactoside and complex carbohydrates.
• In immune regulation, galectin-1 inhibits CD45 protein phosphatase activity, sustaining Lyn kinase phosphorylation, and strongly induces T-cell apoptosis. It also negatively regulates Th17 cell differentiation via activation of the receptor CD69. Related references: PMID:24752896
• The protein is localized to the extracellular space and matrix, as well as the cytoplasm, and is also secreted.
• Galectin-1 is expressed in a wide range of tissues, including placenta (trophoblasts, stromal cells, endothelium), fetal membranes, cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, and hematopoietic cells.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics and direct protein sequencing studies.
• Given its lectin activity and broad tissue expression, galectin-1 may also participate in antimicrobial responses and cell–extracellular matrix interactions.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 15 kDa. Consider using a 15% SDS-PAGE gel and testing lysates from human, mouse, or rat tissues or cell lines.
• For immunofluorescence/ICC, include appropriate permeabilization steps since galectin-1 is found both intracellularly and in the extracellular space. Validate fixation and permeabilization conditions in the relevant sample system.
• When using in ELISA, the antibody can serve as a capture or detection reagent. Note that polyclonal antibodies may show lot-to-lot variation; include appropriate controls and consider cross-reactivity in complex samples.
• If high background or nonspecific binding is observed, especially in tissue immunohistochemistry, optimize blocking conditions and perform antigen retrieval as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering this anti-Galectin-1 antibody alongside a comprehensive portfolio of antibodies, antigens, and ancillary reagents. We support diagnostic manufacturers and researchers with validated antibody pairs, bulk orders, and tailored sourcing for rare targets. Whether you need recombinant proteins, optimized polyclonal antibodies, or technical consultancy, CamelBio streamlines your path from concept to clinic.