Anti-Galectin-1 (LGALS1) Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09382

Core Product Specifications and Parameters

Biological Background: Galectin-1 Function and Localization

• Galectin-1, encoded by the LGALS1 gene, is a member of the galectin family of carbohydrate-binding proteins, also known as 14 kDa laminin-binding protein or beta-galactoside-binding lectin L-14-I.
• Functions as a lectin that binds beta-galactoside and a wide array of complex carbohydrates, playing key roles in regulating apoptosis, cell proliferation, and cell differentiation.
• Inhibits CD45 protein phosphatase activity, thereby preventing dephosphorylation of Lyn kinase, and acts as a strong inducer of T-cell apoptosis.
• Plays a negative role in Th17 cell differentiation through activation of the receptor CD69. Related references: PMID:24752896
• Subcellular localization includes secretion into the extracellular space and matrix, as well as cytoplasmic localization.
• Post-translational modifications include acetylation and phosphorylation, as identified by direct protein sequencing and proteomics.
• Exhibits broad tissue expression, including placenta, decidua, fetal membranes, cardiac, smooth and skeletal muscle, neurons, thymus, kidney, and hematopoietic cells.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1–100 of human Galectin-1; this region may help ensure recognition of the native protein.
• For Western blot applications, note that Galectin-1 has a predicted molecular weight of ~15 kDa; consider using appropriate gel percentages and validate detection using relevant control lysates.
• When performing immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), optimal antibody dilution and antigen retrieval conditions should be determined empirically.
• This antibody has cited cross-reactivity with human, mouse, and rat samples; validate species-specific performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, providing validated antibodies and supporting reagents for diagnostic development and research applications. This anti-Galectin-1 monoclonal antibody aligns with our portfolio focused on apoptosis, immunoregulation, and cell proliferation targets. With capabilities including bulk antibody supply, custom antibody pair validation, and rare target sourcing, CamelBio bridges the gap from concept to clinic for your diagnostic needs.