Anti-GALC Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P54803

Core Product Specifications and Parameters

Biological Background: GALC Function and Localization

• GALC (galactocerebrosidase), also referred to as galactocerebroside beta-galactosidase or galactosylceramidase, is a lysosomal hydrolase involved in sphingolipid metabolism.
• It hydrolyzes the galactose ester bonds of glycolipids, including galactosylceramide and galactosylsphingosine. Related references: PMID:8281145, PMID:8399327
• GALC is responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney, and epithelial cells of the small intestine and colon. Related references: PMID:8281145, PMID:8399327
• The protein is localized in the lysosome.
• GALC is expressed at the protein level in testis, brain, placenta, kidney, and liver, and is detectable in urine.
• Mutations in GALC cause Krabbe disease (globoid cell leukodystrophy), a severe neurodegenerative lysosomal storage disorder.
• The protein is a glycoprotein with disulfide bonds and alternative splicing; it contains a signal peptide for lysosomal targeting.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment encompassing amino acids 40-300 of human GALC, which is expected to generate antibodies targeting the N-terminal region. Due to sequence conservation, cross-reactivity with mouse and rat GALC is plausible.
• For Western blot (WB), the predicted molecular weight of GALC is approximately 77 kDa; a specific band near this size is anticipated.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and antibody dilution. Lysosomal localization may appear as punctate cytoplasmic staining.
• In ELISA applications, this antibody may be utilized as a capture or detection reagent; validate assay conditions in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider of IVD raw materials, offering diagnostic manufacturers and researchers reliable access to antibodies like anti-GALC for lysosomal storage disease research. With a comprehensive portfolio including validated antibody pairs and bulk ancillary reagents, we support every stage from assay development to commercialization. Contact our team to discuss your specific sourcing needs for rare targets and customized solutions.