Anti-Galactosidase alpha (GLA) Polyclonal Antibody for WB, ELISA - P06280

Core Product Specifications and Parameters

Biological Background: Alpha-galactosidase A (GLA) Function and Localization

• The GLA gene encodes alpha-galactosidase A, also referred to as alpha-D-galactosidase A, galactosylgalactosylglucosylceramidase GLA, and melibiase.
• Alpha-galactosidase A is a lysosomal enzyme that catalyzes the hydrolysis of glycosphingolipids, playing a critical role in lipid metabolism and lysosomal degradation.
• It specifically cleaves terminal alpha-galactose residues from substrates such as globotriaosylceramide (Gb3) and other glycoconjugates.
• The enzyme localizes to the lysosome, where it functions optimally at an acidic pH.
• Mutations in GLA are linked to Fabry disease, an X-linked lysosomal storage disorder characterized by the accumulation of glycosphingolipids (keyword: Disease variant).
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are important for enzyme stability and activity (keywords: Glycoprotein, Disulfide bond).
• The protein is synthesized with a signal peptide that directs it to the endoplasmic reticulum for subsequent processing and trafficking to the lysosome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 150–429 of human GLA, encompassing most of the catalytic domain; this antibody may recognize both mature and denatured forms, and is suitable for WB and ELISA.
• For Western blotting, a band at approximately 49 kDa (glycosylated form) is expected; proper lysate preparation and reducing conditions may be important.
• For ELISA, consider using recombinant GLA protein or cellular extracts as standards/antigens, and validate detection in your specific assay format.
• Due to lysosomal localization, cell or tissue fractionation may aid in signal enrichment.
• Cross-reactivity with other species has not been extensively tested; validation is advised if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we bridge the gap between diagnostic developers and high-quality raw materials. This anti-GLA polyclonal antibody supports research in lysosomal storage disorders like Fabry disease, and we provide a complete portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare targets such as GLA, our sourcing expertise ensures reliable access to the critical components you need for your IVD assay development.