Anti-GAL3ST4 Rabbit Polyclonal Antibody for WB and ELISA - Q96RP7

Core Product Specifications and Parameters

Biological Background: GAL3ST4 Function and Localization

• Catalyzes the transfer of a sulfate group from PAPS to the C-3 hydroxyl group of terminal non-reducing beta-1,3-linked galactose residues, exclusively in O-linked glycoproteins, acting on core 1 (Gal-beta-1,3-GalNAc-R) structures. Related references: PMID:11333265 PMID:14701868
• In vitro, also recognizes Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc-R, asialofetuin, the Globo H precursor and Gal-beta-1,3(6-O-sulfo)GalNAc-R structures as good substrates. Related references: PMID:11333265 PMID:14701868
• Localized to the Golgi apparatus, specifically the Golgi stack membrane, consistent with its role in post-translational sulfation of glycoproteins.
• Expressed mainly in placenta, thymus, testis, ovary, spinal cord, trachea, and adrenal gland, with lower levels in brain, lung, spleen, prostate, small intestine, colon, stomach, thyroid, and lymph node.
• Contains a signal-anchor and a transmembrane helix; undergoes alternative splicing and glycoprotein modifications.
• The mature protein has a calculated molecular weight of approximately 54 kDa, consistent with the observed band in Western blotting.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 54 kDa; optimize blocking conditions and antibody dilution for your specific sample type.
• The immunogen covers amino acids 137–486, providing broad epitope coverage; consider this when assessing isoform detection.
• This polyclonal antibody may be suitable for ELISA; validate in the relevant sample matrix with appropriate titration.
• Cross-reactivity with mouse and rat is reported; perform sequence alignment and confirm species-specific reactivity in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories. Whether you require antibodies targeting golgi sulfotransferases like GAL3ST4 or other rare targets, our team can streamline procurement and provide technical consultation to support your assay development from concept to clinic.