Anti-GADD45G Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95257

Core Product Specifications and Parameters

Biological Background: GADD45G Function and Localization

• GADD45G, also known as Growth arrest and DNA damage-inducible protein GADD45 gamma, is a member of the GADD45 family of stress-responsive proteins. Alternative names include Cytokine-responsive protein CR6 and DNA damage-inducible transcript 2 protein (DDIT-2).
• The protein is involved in the regulation of cell growth and apoptosis, acting as a crucial mediator of stress responses.
• It mediates activation of the stress-responsive MTK1/MEKK4 MAP kinase kinase kinase, leading to downstream signaling events.
• Annotation keywords highlight its role in apoptosis, development, and differentiation, suggesting multifaceted biological functions.
• The protein is part of the human reference proteome, and its 3D structure has been partially determined, offering insights into its molecular mechanisms.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to a recombinant fragment covering amino acids 90–159 of human GADD45G. This region may include functional domains; therefore, the antibody is expected to recognize the full-length native protein, but researchers should confirm specificity in their experimental system.
• For Western blotting, a detected band at approximately 17 kDa is consistent with the predicted molecular weight of GADD45G. Consider using appropriate positive control lysates (e.g., induced or stressed human cells) to validate reactivity.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval conditions may significantly affect staining. It is advisable to optimize retrieval buffers and incubation times for the specific tissue type.
• For ELISA applications, the antibody can be used as a capture or detection reagent. Titration experiments are recommended to determine the optimal working concentration.
• Cross-reactivity has been observed with mouse and rat samples, indicating that the target epitope is conserved across these species. When using non-human samples, verify that the antibody performance meets the required sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supporting diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. For researchers focusing on apoptosis, stress signaling, and developmental biology, this anti-GADD45G antibody is one of many validated tools we can supply. Explore our portfolio for optimized monoclonal/polyclonal antibodies, matched antibody pairs, bulk ancillary reagents, and custom sourcing of rare-target raw materials to accelerate your assay development.