Anti-GADD45A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24522

Core Product Specifications and Parameters

Biological Background: GADD45A Function and Localization

• GADD45A, also known as DDIT-1 or GADD45, is a growth arrest and DNA damage-inducible protein that plays critical roles in cellular stress responses.
• It functions as a regulator of p38 MAPKs by inhibiting p38 phosphorylation and activity, a mechanism inferred from sequence similarity.
• GADD45A may modulate PCNA interaction with certain CDK complexes, contributing to its role in DNA repair.
• It stimulates DNA excision repair in vitro and inhibits entry of cells into S phase, thereby linking DNA damage sensing to cell cycle control.
• The protein is predominantly localized in the nucleus, consistent with its functions in DNA repair and cell cycle regulation.
• GADD45A is a phosphoprotein and is subject to alternative splicing, yielding multiple isoforms.
• Keywords associated with this target include cell cycle, DNA damage, growth arrest, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-165) of human GADD45A, suggesting potential recognition of full-length protein and possibly some isoforms.
• For WB, a band around 18 kDa is expected; consider using appropriate molecular weight markers and validating with positive controls.
• In IHC-P applications, validate antibody specificity using known expressing tissues and include proper antigen retrieval methods.
• For ELISA, the antibody can be explored as capture or detection reagent; optimal pairing conditions should be determined empirically.
• Cross-reactivity with mouse and rat has been reported; verify in your specific model system to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this anti-GADD45A antibody as part of our comprehensive IVD raw material portfolio, supporting DNA damage and cell cycle research. We provide validated antibody pairs, bulk quantities of monoclonal/polyclonal antibodies, and ancillary reagents to streamline diagnostic development. For rare or specialized targets, our sourcing solutions help you accelerate from concept to clinic.