Anti-GAD67/GAD1 Rabbit Monoclonal Antibody for WB and ELISA - Q99259

Core Product Specifications and Parameters

Biological Background: GAD67/GAD1 Function and Localization

• Glutamate decarboxylase 1 (GAD67) is the primary enzyme responsible for synthesizing the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) in the central nervous system, using pyridoxal 5'-phosphate as a cofactor.
• Notably, the purified GAD67 enzyme is reported to be enzymatically inactive as a glutamate decarboxylase, suggesting regulation by autoinhibition or post-translational modification.
• The GAD1 gene undergoes alternative splicing, producing multiple isoforms that may have distinct cellular roles.
• GAD67 is a phosphoprotein, with phosphorylation potentially modulating its activity and subcellular targeting.
• Mutations in GAD1 are linked to neurological disorders, including epilepsy and intellectual disability.
• Expression of GAD67 is highest in the brain, where it is critical for inhibitory neurotransmission, but it is also detected in pancreatic islets, testis, and adrenal cortex.
• GAD67 belongs to the group II decarboxylase family and exhibits pyridoxal phosphate-dependent lyase activity.

Experimental Guidance and Technical Tips

• The antibody was raised against the N-terminal region (aa 1-100), so it may recognize full-length GAD67 and N-terminal fragments; validate specificity in your experimental system.
• For Western blot, consider using reducing conditions and load an appropriate amount of protein; the expected band is around 67 kDa.
• In ELISA, test both direct and indirect coating strategies with recombinant or native GAD67 to optimize signal-to-noise ratio.
• Cross-reactivity with human, mouse, and rat has been reported; verify performance in your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This anti-GAD67/GAD1 antibody supports research in neuroscience, neurotransmitter metabolism, and neurological disorder diagnostics. Contact us for customized bulk orders and technical consulting to accelerate your assay development.