Anti-GAD65/GAD2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q05329

Core Product Specifications and Parameters

Biological Background: GAD65/GAD2 Function and Localization

• Glutamate decarboxylase 2 (GAD2), also known as GAD-65 or 65 kDa glutamic acid decarboxylase, is a pyridoxal phosphate-dependent enzyme that catalyzes the conversion of glutamate to GABA.
• GAD65 is essential for the synthesis of the major inhibitory neurotransmitter GABA in the central nervous system, modulating neuronal excitability and synaptic transmission.
• The protein is found in the cytoplasm and associates with membranes of the Golgi apparatus, cytoplasmic vesicles, and the presynaptic cell membrane.
• Post-translational modifications include palmitoylation and phosphorylation, which are critical for subcellular targeting and regulation of enzyme activity.
• As a peripheral membrane protein, GAD65 cycles between cytosolic and membrane-bound states, a process linked to synaptic vesicle dynamics.
• Consistent with its role in neurotransmission, GAD65 is expressed primarily in GABAergic neurons.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 401-585) of human GAD65, which likely contains conserved epitopes, consistent with reported cross-reactivity to mouse and rat.
• For Western blotting, expect a band at ~65 kDa; consider using brain tissue lysates or neuronal cell lines known to express GAD65.
• In immunofluorescence (IF-P), permeabilize cells to detect both cytoplasmic and membrane-associated pools; co-staining with synaptic markers can confirm presynaptic localization.
• As a polyclonal antibody, validate lot-to-lot consistency in your specific assay system to ensure reproducibility.
• The antibody is applicable to ELISA; optimize coating and detection conditions based on your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and recombinant proteins. For targets like GAD65, critical in neuroscience and autoantibody diagnostics, we offer high-quality polyclonal antibodies alongside supporting reagents and technical consultation to streamline assay development. Let CamelBio be your trusted sourcing bridge from concept to clinic.