Anti-GAD65/GAD2 Mouse Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q05329

Core Product Specifications and Parameters

Biological Background: GAD65/GAD2 Function and Localization

• GAD65/GAD2 encodes glutamate decarboxylase 2, also known as the 65 kDa glutamic acid decarboxylase (GAD-65).
• It catalyzes the production of GABA, the major inhibitory neurotransmitter.
• Subcellularly, it is found in the cytoplasm, cytosol, cytoplasmic vesicles, presynaptic cell membrane, and Golgi apparatus membrane.
• The protein is a pyridoxal phosphate (PLP)-dependent decarboxylase.
• It undergoes palmitoylation (lipoprotein) and phosphorylation, which are likely to influence its membrane association and activity.
• GAD65 is involved in neurotransmitter biosynthesis at synapses.

Experimental Guidance and Technical Tips

• As the antibody was generated using a recombinant human GAD65/GAD2 immunogen, it is expected to detect both native and denatured GAD65. For Western blotting, a band around 65 kDa should be observed.
• Given the cytoplasmic and membrane-associated localization of GAD65, appropriate permeabilization and antigen retrieval steps should be optimized for immunofluorescence (IF-P) and immunohistochemistry (IHC-P).
• Cross-reactivity with mouse and rat has been reported by the supplier; however, validation in the specific tissue or cell system is recommended.
• For ELISA applications, consider using the antibody as a capture or detection reagent in sandwich ELISA setups, paired with another GAD65-specific antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with end-to-end access to IVD raw materials and technical consulting. This anti-GAD65/GAD2 antibody serves as a reliable tool for studies into GABAergic neurotransmission and related biomarker development. Complementing our antibody portfolio, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expert assistance in sourcing reagents for rare targets, ensuring a seamless transition from concept to clinical application.