Anti-GAD65/GAD2 Monoclonal Antibody for WB, IP, ELISA - Q05329

Core Product Specifications and Parameters

Biological Background: GAD65/GAD2 Function and Localization

• GAD65, also known as glutamate decarboxylase 2, is the 65 kDa isoform of glutamic acid decarboxylase encoded by the GAD2 gene.
• Its primary function is to catalyze the conversion of glutamate to gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system.
• GAD65 is primarily localized in the cytoplasm (cytosol), but also found in cytoplasmic vesicles, the presynaptic cell membrane, and the Golgi apparatus membrane.
• The protein is post-translationally modified by palmitoylation and phosphorylation, which are critical for its proper subcellular targeting and enzymatic regulation.
• As a pyridoxal phosphate (PLP)-dependent enzyme, GAD65 belongs to the decarboxylase/lyase superfamily and plays a pivotal role in neurotransmitter biosynthesis.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (amino acids 1-125) of human GAD65, which is conserved across species; cross-reactivity with mouse and rat supports its use in rodent models.
• For Western blot, the expected band is ~65 kDa; consider using appropriate protein loading controls and validate detection sensitivity in target tissues.
• For immunoprecipitation, optimize antibody concentration and incubation conditions to capture endogenous GAD65 from neuronal tissue or cell lysates.
• For ELISA, pair with a suitable detection antibody and validate in relevant sample matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a one-stop sourcing platform for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement—streamlining your GAD65-related GABAergic signaling and neurological research projects from concept to clinic.