Anti-GABRR1 Rabbit Polyclonal Antibody for WB and ELISA - P24046

Core Product Specifications and Parameters

Biological Background: GABRR1 Function and Localization

• GABRR1 encodes the gamma-aminobutyric acid receptor subunit rho-1 (GABA(A) receptor subunit rho-1), a subunit of pentameric ligand-gated chloride channels that mediate inhibitory neurotransmission in the brain.
• As a rho subunit, it forms homomeric or heteromeric GABA-gated chloride channels (formerly known as GABA(C) receptors) that are insensitive to bicuculline and benzodiazepines, with GABA binding at rho intersubunit interfaces.
• When activated by GABA, rho-1-containing receptors allow chloride ion influx, hyperpolarizing the postsynaptic membrane. Related references: PMID:37659407
• Rho-1 subunits are highly expressed in the retina, where they may play a role in retinal neurotransmission. They are also detected at lower levels in brain, lung, and thymus. Related references: PMID:1849271
• In the cerebellum, rho-1 contributes to tonic and phasic inhibition in Purkinje neurons and may regulate glial development through extrasynaptic transmission.
• The protein localizes to the postsynaptic cell membrane.
• Key post-translational modifications include glycosylation and disulfide bond formation; the predicted molecular weight is ~56 kDa.
• Alternative splicing generates different isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic C-terminal region (aa 365-465) of human GABRR1. Ensure target protein is denatured and reduced for optimal detection in WB.
• Expect a band near 56 kDa; however, N-linked glycosylation may result in a slightly higher apparent molecular weight. Use appropriate molecular weight markers.
• Validate antibody specificity in the relevant sample system (e.g., retina or brain lysates) by including positive and negative controls.
• For ELISA, this antibody can be used for antigen capture or detection; titrate to determine optimal working concentration.
• Cross-reactivity with mouse and rat is indicated; however, confirm in corresponding species' tissues if used for IHC or other applications not originally validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your diagnostic and research developments with comprehensive sourcing solutions for IVD raw materials. For GABA receptor and neurotransmission research, we provide polyclonal antibodies like anti-GABRR1, along with validated antibody pairs, optimized monoclonal alternatives, bulk ancillary reagents, and customized rare-target raw-material sourcing to accelerate your project from concept to clinic.