Anti-GABRG1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N1C3

Core Product Specifications and Parameters

Biological Background: GABRG1 Function and Localization

• Encodes the gamma-1 subunit of the heteropentameric GABA(A) receptor, a ligand-gated chloride channel (gene: GABRG1).
• Mediates fast inhibitory neurotransmission in the brain by gating chloride ions in response to GABA binding, reducing postsynaptic excitability. Related references: PMID:10449790
• The receptor complex contains a central pore and has GABA active binding sites at alpha-beta subunit interfaces, with the gamma subunit modulating benzodiazepine pharmacology.
• Localizes primarily to the postsynaptic cell membrane and, more broadly, the cell membrane.
• Classified as a transmembrane, disulfide bond-containing glycoprotein and lipoprotein, with palmitoylation sites.
• Belongs to the ligand-gated ion channel superfamily and contributes to chloride transport across the synapse.
• Molecular weight annotated at ~53.6 kDa (UniProt), consistent with SDS-PAGE detection around 54 kDa.

Experimental Guidance and Technical Tips

• The immunogen targets a cytoplasmic loop region (a.a. 200–260), enabling detection of total GABRG1 in whole-cell lysates without permeabilization.
• For Western blot, validate the observed band size (expected ~54 kDa) in human, mouse, or rat brain tissue; consider loading controls to confirm specificity.
• For IF/ICC, optimize fixation and permeabilization conditions; check membrane-associated staining patterns consistent with synaptic localization.
• ELISA applications may require pairing with a detection antibody targeting a distinct epitope; test on recombinant protein or native extracts.
• Cross-reactivity with human, mouse, and rat allows translational neuroscience research; however, verify expression levels in your model system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio supports your diagnostic and research programs with seamless access to high-quality antibodies, reagents, and technical expertise—from early discovery through clinical application. Our portfolio covers neuroscience targets like GABRG1, with validated antibody pairs, optimized polyclonals, and custom sourcing for rare subunit-specific tools. Rely on CamelBio for bulk ancillary reagents and one-on-one consultation to accelerate your assay development.