Anti-GABRD Rabbit Polyclonal Antibody for WB, ELISA - O14764

Core Product Specifications and Parameters

Biological Background: GABRD Function and Localization

• Gamma-aminobutyric acid receptor subunit delta (GABRD) is a subunit of the heteropentameric GABA(A) receptors, which are ligand-gated chloride channels gated by the major inhibitory neurotransmitter GABA. When activated, GABAARs selectively allow chloride ion flow, mediating fast inhibitory synaptic transmission. Related references: PMID:35355020
• As a delta subunit, GABRD-containing receptors are predominantly located in extrasynaptic or perisynaptic positions, particularly in hippocampus and cerebellar granule cells, and contribute to tonic GABAergic inhibition, regulating neuronal excitability.
• The GABA(A) receptor complex consists of five subunits arranged around a central pore; GABA binding sites reside at alpha-beta subunit interfaces. GABRD likely partners with alpha and beta subunits to form functional channels.
• GABRD is a transmembrane glycoprotein with a disulfide bond, and undergoes phosphorylation, as indicated by its annotation as a glycoprotein and phosphoprotein.
• Subcellularly, GABRD localizes to the cell membrane, consistent with its role as an ion channel receptor.
• Functional studies indicate that alpha-4-beta-3-delta GABAARs can simultaneously bind GABA and histamine, with histamine binding at the interface of two neighboring beta subunits, potentially regulating sleep and wakefulness. Related references: PMID:35355020
• Clinically, mutations in GABRD are associated with epilepsy and other disease variants, highlighting its importance in neurological function.
• GABRD is also known by alternative names such as GABA(A) receptor subunit delta and gene aliases EJM7, EIG10, GEFSP5.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 31-248 of human GABRD, corresponding to the N-terminal extracellular domain. This design targets a region that is accessible in the native receptor and may be suitable for detecting both denatured and native protein.
• For Western blot, the predicted molecular weight is 51 kDa; however, due to glycosylation and other post-translational modifications, the observed band may migrate at a higher apparent molecular weight. Consider using deglycosylation enzymes if precise size determination is needed.
• The antibody cross-reacts with rat GABRD, implying sequence homology in the immunogen region between human and rat. Researchers may also evaluate cross-reactivity with other species based on sequence alignment.
• For ELISA applications, the antibody can potentially be used as a capture or detection reagent for recombinant GABRD protein or enriched receptor preparations. Titration and buffer optimization are recommended.
• Given the role of GABRD in tonic inhibition, this antibody may be useful in immunohistochemistry or immunocytochemistry after appropriate validation, though only WB and ELISA are listed in the product specifications; alternative applications require individual optimization.

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At CamelBio, we streamline your IVD raw material procurement by offering a comprehensive range of high-quality antibodies, antigens, and ancillary reagents for neuroscience and neurological disorder research. Whether you need validated antibody pairs, bulk monoclonal/polyclonal antibodies, or rare target sourcing, our one-stop platform ensures reliable supply to accelerate your diagnostic development. Explore our GABRD polyclonal antibody and complementary products to support your tonic inhibition studies and epilepsy biomarker programs.