Anti-GABRA5 Rabbit Polyclonal Antibody for WB and ELISA - P31644

Core Product Specifications and Parameters

Biological Background: GABRA5 Function and Localization

• GABRA5 encodes the alpha-5 subunit of the GABA(A) receptor, a heteropentameric ligand-gated chloride channel that mediates fast inhibitory neurotransmission in the brain. Related references: PMID:14993607 PMID:29961870 PMID:30140029

• GABA(A) receptors assemble as pentamers around a central pore, with the GABA binding site formed at the interface of alpha and beta subunits. Related references: PMID:30140029

• Upon activation by GABA, GABRA5-containing receptors selectively permit chloride ion flux across the membrane, driving postsynaptic hyperpolarization. Related references: PMID:14993607 PMID:30140029

• Receptors containing GABRA5 are predominantly extrasynaptic and are key mediators of tonic GABAergic inhibition in the hippocampus.

• Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons are critically involved in learning and memory processes.

• The protein localizes to the postsynaptic cell membrane and general cell membrane, consistent with its role in synaptic and extrasynaptic signaling.

• Mutations in GABRA5 are associated with developmental and epileptic encephalopathy (DEE79/EIEE79), highlighting its clinical significance in neurological disease.

• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination, which may regulate receptor trafficking and stability.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 32–259, spanning most of the extracellular N-terminal domain; this region contains critical epitopes for subunit-specific recognition.
• For Western blot, the predicted molecular weight is approximately 52 kDa; validate specificity using appropriate positive and negative control lysates (e.g., brain tissue from wild-type vs. GABRA5 knockout models).
• Cross-reactivity with mouse and rat species enables translational studies; confirm performance in the target tissue and experiment type before large-scale use.
• In ELISA, consider using the antibody for capture or detection of recombinant GABRA5; pairing with a matched detection antibody or direct labeling may be required.
• Buffer and blocking conditions should be optimized empirically, particularly for membrane protein detection; avoid harsh denaturing conditions that may disrupt conformational epitopes.

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CamelBio specializes in supplying diagnostic manufacturers and research laboratories with comprehensive IVD raw-material solutions, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare target sourcing. For neuroscience and ion channel research, we provide dependable access to reagents like anti-GABRA5 antibodies, supported by technical consultation and flexible procurement options to streamline your assay development.