Anti-GABRA5 Polyclonal Antibody for WB, ELISA - P31644

Core Product Specifications and Parameters

Biological Background: GABRA5 Function and Localization

• Gamma-aminobutyric acid receptor subunit alpha-5 (GABA(A) receptor subunit alpha-5), encoded by the GABRA5 gene, is an integral membrane protein that constitutes the alpha subunit of the heteropentameric GABA-gated chloride channel. Related references: PMID:14993607, PMID:29961870, PMID:30140029
• GABA(A) receptors (GABAARs) are assembled from five subunits surrounding a central pore, with GABA binding sites located at the interface of alpha and beta subunits. Related references: PMID:30140029
• Upon GABA binding, GABRA5-containing GABAARs facilitate the selective influx of chloride ions across the postsynaptic membrane, leading to neuronal hyperpolarization and inhibition of neurotransmission. Related references: PMID:14993607, PMID:30140029
• GABRA5 subunits are predominantly located at extrasynaptic sites in the hippocampus, where they mediate tonic GABAergic inhibition and contribute to cognitive processes such as learning and memory.
• The subunit is a glycoprotein with disulfide bond formation in the extracellular domain and undergoes post-translational modifications including ubiquitination (Ubl conjugation).
• Mutations in GABRA5 are associated with developmental and epileptic encephalopathy (DEE79, EIEE79), underscoring its critical role in neurological function.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular region (aa 32-259) of human GABRA5, providing recognition of both native and denatured protein conformations; confirm performance in your specific assay system.
• For Western blotting, brain tissue lysates (particularly from the hippocampus) are recommended as positive controls due to high GABRA5 expression.
• In ELISA applications, use the recombinant protein immunogen as a reference standard to assess antibody binding.
• Cross-reactivity with mouse and rat GABRA5 is reported; consider sequence alignment verification if using these species.
• As GABRA5 is a transmembrane protein, optimized lysis and sample preparation protocols may be required for efficient extraction.
• Validate antibody specificity using siRNA knockdown, knockout tissue, or blocking peptide controls to exclude off-target binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consultancy, simplifying development from concept to clinic. Our portfolio includes high-quality polyclonal and monoclonal antibodies like this anti-GABRA5 antibody, ideal for neuroscience research and IVD assay development. From validated antibody pairs to bulk ancillary reagents and rare target sourcing, CamelBio supports your entire project with reliable, one-stop supply solutions.