Anti-GABRA3 Rabbit Polyclonal Antibody for WB and ELISA - P34903

Core Product Specifications and Parameters

Biological Background: GABRA3 Function and Localization

• GABRA3 encodes the alpha-3 subunit of the GABA(A) receptor, a heteropentameric ligand-gated chloride channel that mediates fast inhibitory synaptic transmission in the brain.
• Upon binding of GABA, the receptor opens an intrinsic chloride channel, allowing chloride influx that hyperpolarizes the postsynaptic membrane and reduces neuronal excitability. Related references: PMID:16412217, PMID:29053855
• The GABA(A) receptor is typically composed of two alpha, two beta, and one gamma subunit, with the GABA binding site located at the interface of alpha and beta subunits.
• GABRA3 is primarily expressed in the central nervous system, with localization to postsynaptic cell membranes.
• The protein undergoes post-translational modifications including N-glycosylation and phosphorylation, as indicated by keywords glycoprotein and phosphoprotein.
• GABRA3 has been associated with neurological disorders such as epilepsy, supported by the disease variant keyword.
• The predicted molecular weight of the GABRA3 protein is approximately 55 kDa, consistent with the provided antibody immunogen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 29-276), which may be useful for detecting the mature receptor or cleavage products.
• Consider using this antibody in WB with brain tissue lysates where GABRA3 is highly expressed.
• For ELISA, consider coating with recombinant antigen or native receptor complexes.

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CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For neuroscience and ion channel research, CamelBio supports sourcing of rare targets like GABRA3 to accelerate assay development from concept to clinic.