Anti-GABRA2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P47869

Core Product Specifications and Parameters

Biological Background: GABRA2 Function and Localization

• GABRA2 encodes the alpha-2 subunit of the heteropentameric GABA(A) receptor, a ligand-gated chloride channel that mediates fast inhibitory neurotransmission in the brain. Related references: PMID:10449790 PMID:29961870 PMID:31032849
• Upon GABA binding at the alpha-beta subunit interface, the channel opens to selectively allow chloride ion influx, causing postsynaptic membrane hyperpolarization and reducing neuronal excitability.
• The alpha-2 subunit exhibits synaptogenic activity in combination with beta-2 subunits, while gamma-2 is necessary but not sufficient for rapid synaptic contact formation.
• GABRA2 is localized to the postsynaptic cell membrane, cytoplasmic vesicle membrane, and dendrites, consistent with its role in synaptic inhibition.
• As a glycoprotein with disulfide bonds, GABRA2 undergoes post-translational modifications typical of neurotransmitter receptors and is classified under the ligand-gated ion channel family.
• Mutations in GABRA2 are associated with developmental and epileptic encephalopathies (DEE78, EIEE78), highlighting its critical role in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic loop region (amino acids 300–400) which is relatively conserved between human and mouse, supporting the observed cross-reactivity.
• For Western blotting, an expected band at approximately 51 kDa should be detected; consider using brain tissue lysates or GABA(A) receptor-enriched membrane preparations.
• In immunohistochemistry (paraffin), antigen retrieval may be required; validate staining in appropriate positive control tissues such as brain sections.
• For ELISA, the synthetic peptide can serve as coating antigen; optimal coating concentration and detection conditions should be determined empirically.
• Always include appropriate negative controls (e.g., peptide blocking, knockout tissue) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting—from early concept to clinical application. For neuroscience and epilepsy-focused projects targeting GABRA2, we offer a range of validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to streamline your assay development and supply chain. Our team also supports rare-target raw material sourcing, ensuring you can advance your diagnostic pipeline efficiently.