Anti-GABRA1 Polyclonal Antibody for WB, ELISA - P14867

Core Product Specifications and Parameters

Biological Background: GABRA1 Function and Localization

• GABRA1 encodes the alpha-1 subunit of the gamma-aminobutyric acid type A (GABA(A)) receptor, a ligand-gated chloride channel responsible for fast inhibitory neurotransmission in the central nervous system (PubMed:23909897, PubMed:25489750, PubMed:29950725). Related references: PMID:23909897 PMID:25489750 PMID:29950725

• The GABA(A) receptor is a heteropentamer composed of five subunits arranged around a central pore, with GABA binding occurring at the interface of alpha and beta subunits (PubMed:29950725, PubMed:30602789). Related references: PMID:29950725 PMID:30602789

• Upon GABA binding, the receptor selectively conducts chloride ions across the postsynaptic membrane, leading to neuronal hyperpolarization and reduced action potential generation (PubMed:23909897, PubMed:29950725, PubMed:30602789). Related references: PMID:23909897 PMID:29950725 PMID:30602789

• Alpha-1-containing GABA(A) receptors are predominantly synaptic and play a critical role in synapse formation and plasticity, particularly in the orbitofrontal cortex regulating context-dependent action selection (By similarity).

• The GABRA1 subunit is a glycoprotein with conserved disulfide bonds and transmembrane helices, and it localizes to the postsynaptic cell membrane, as well as cytoplasmic vesicle membranes.

• Mutations in GABRA1 are linked to various epilepsy syndromes, including early infantile epileptic encephalopathy (DEE19) and juvenile myoclonic epilepsy (EJM), highlighting its essential role in neuronal excitability.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 28-251 of human GABRA1, NP_000797.2) targets the extracellular N‑terminal domain, which may favor detection of native or denatured protein under appropriate conditions.
• For Western blot (WB), validate antibody performance in brain tissue lysates from human, mouse, or rat, and consider loading controls to confirm specificity.
• For ELISA, test serial dilutions of the antibody against recombinant target protein or native antigen, and optimize coating and blocking conditions.
• Because GABRA1 is a transmembrane protein, consider mild denaturing conditions if detection of the 52 kDa band is weak.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, supporting diagnostic manufacturers and research laboratories with a wide array of validated reagents. This GABRA1 polyclonal antibody is part of our neuroscience portfolio, complementing our offerings of antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Let CamelBio streamline your supply chain from discovery to clinical application.