Anti-GABPB2 Polyclonal Antibody for WB, ELISA - Q8TAK5

Core Product Specifications and Parameters

Biological Background: GABPB2 Function and Localization

• GABPB2 (GA-binding protein subunit beta-2) is a transcription factor that may specifically bind purine-rich GA repeats in DNA.
• It is a component of the heterodimeric GABP complex, where the beta subunit contributes to DNA-binding specificity and transcriptional activation.
• Structurally, GABPB2 contains ankyrin (ANK) repeats and a coiled-coil domain, facilitating protein-protein interactions and complex formation.
• The protein localizes predominantly to the nucleus, consistent with its role in regulating gene expression.
• Post-translational modifications include phosphorylation, which may modulate its activity in response to cellular signals.
• Identified through proteomics and part of the human reference proteome, GABPB2 is implicated in fundamental transcriptional regulatory networks.
• Alternative names include GABPB-2, reflecting its close relationship with other GABP family members.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 239-448 of human GABPB2; this C-terminal region may be conserved across species; validate reactivity with mouse and rat samples.
• For Western blot (WB), anticipate a band near 49 kDa; consider using positive controls expressing GABPB2 and optimize blocking and detection conditions.
• In ELISA, titrate the antibody to determine optimal concentrations for coating and detection; note that polyclonal antibodies may exhibit batch-specific affinities.
• Since this is a polyclonal antibody, it may recognize multiple epitopes; confirm specificity with knockdown or overexpression experiments.
• Store as recommended and avoid repeated freeze-thaw cycles to maintain activity.

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CamelBio provides comprehensive sourcing for IVD raw materials, including validated antibodies like anti-GABPB2 for transcription factor research. Our portfolio encompasses optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing to support diagnostic development from concept to clinic. With expert technical consultation, we help streamline your assay development for research and clinical applications.