Anti-GABPB1 Polyclonal Antibody for WB and ELISA - Q06547

Core Product Specifications and Parameters

Biological Background: GABPB1 Function and Localization

• GA-binding protein subunit beta-1 (GABPB1) is a transcription factor that interacts with purine-rich repeats (GA repeats) to regulate gene expression. Related references: PMID:10675337 PMID:8441384 PMID:8816484
• GABPB1 acts as a master regulator of nuclear-encoded mitochondrial genes, coordinating mitochondrial biogenesis and function.
• During adenovirus infection, GABPB1 is necessary for the expression of the viral E4 gene.
• The protein contains ANK repeats, which mediate protein-protein interactions, and is subject to acetylation and alternative splicing.
• GABPB1 is localized to the nucleus, consistent with its role in transcriptional regulation.
• Alternative splicing generates multiple isoforms, including GABPB-2, E4TF1-47, and E4TF1-53, which may have distinct functional properties.

Experimental Guidance and Technical Tips

• Consider validating the antibody in your specific sample system, as polyclonal antibodies may exhibit batch-to-batch variability.
• The immunogen is a recombinant fragment corresponding to amino acids 231-395 of human GABPB1, targeting the C-terminal region; this region may be conserved across isoforms.
• For Western blot (WB), the predicted molecular weight is approximately 42 kDa; however, additional bands may be observed due to post-translational modifications or alternative splicing.
• In ELISA applications, optimize coating antigen concentration and antibody titration to achieve optimal signal-to-noise ratio.
• Cross-reactivity with mouse enables studies in murine models; verify species-specific reactivity if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, all tailored for the transcription factor and mitochondrial research area. We also specialize in sourcing rare-target raw materials, ensuring your GABPB1-related projects are fully supported.