Anti-GABPA Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q06546

Core Product Specifications and Parameters

Biological Background: GABPA Function and Localization

• GA-binding protein alpha chain (GABPA), also known as nuclear respiratory factor 2 subunit alpha (NRF2A) or transcription factor E4TF1-60, is a key transcriptional regulator.
• It functions as a transcription factor that specifically binds to purine-rich GA repeat sequences in target gene promoters.
• GABPA positively regulates the transcription of the transcriptional repressor RHIT/ZNF205, contributing to gene regulatory networks. Related references: PMID:22306510
• During adenovirus infection, GABPA is necessary for the expression of the viral E4 gene.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• GABPA is subject to phosphorylation, as indicated by phosphoproteomic studies, and is classified as a DNA-binding phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen consists of amino acids 1–260 of human GABPA, covering the N-terminal region; this design may confer specificity for full-length and N-terminal fragment detection.
• For western blotting, consider using nuclear extracts from HeLa, HEK293, or other common human cell lines as positive controls, given the ubiquitous nuclear expression.
• In immunohistochemistry (IHC-P), validate antigen retrieval conditions (e.g., citrate buffer, pH 6.0) on relevant tissue sections, and include isotype controls.
• Cross-reactivity with mouse and rat implies possible utility across rodent models, but confirm sequence homology if epitope resolution is critical.
• ELISA applications may require careful titration and pairing with a matched detection antibody if quantitative assays are developed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to high-quality IVD raw materials, including validated antibodies and recombinant proteins. This anti-GABPA polyclonal antibody supports studies in transcriptional regulation, host-virus interactions, and nuclear signaling. For bulk orders, custom antibody development, or complementary reagents, our team offers comprehensive sourcing solutions to accelerate your project from concept to clinic.