Anti-GABPA Polyclonal Antibody for WB, ELISA - Q06546

Core Product Specifications and Parameters

Biological Background: GA-binding protein alpha chain Function and Localization

• GA-binding protein alpha chain (GABPA), also known as nuclear respiratory factor 2 subunit alpha (NRF2α) and transcription factor E4TF1-60, is a human transcription factor encoded by the GABPA gene (Gene ID: 2551).
• GABPA binds to purine-rich repeats (GA repeats) and positively regulates transcription of target genes, including the transcriptional repressor RHIT/ZNF205. Related references: PMID:22306510
• During microbial infection, GABPA is necessary for the expression of the Adenovirus E4 gene.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modification: GABPA is annotated as a phosphoprotein, suggesting regulation by phosphorylation.
• According to the UniProt reviewed entry, the calculated molecular weight of GABPA is 51295 Da (~51.3 kDa), which aligns with the observed 51 kDa band.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide spanning amino acids 50-150 of human GABPA. For Western blotting, consider using nuclear protein extracts and validate the signal at approximately 51 kDa.
• This polyclonal antibody is suitable for ELISA; optimal coating or detection concentrations should be determined empirically.
• Reported cross-reactivity with human and rat; performance in other species must be verified.
• As GABPA is a DNA-binding transcription factor, avoid excessive sample heating and consider adding reducing agents during sample preparation to maintain solubility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects IVD manufacturers and laboratories with a comprehensive portfolio of raw materials for transcription factor research, including antibodies against targets like GABPA. From validated antibody pairs and bulk ancillary reagents to customized monoclonal/polyclonal antibody development, CamelBio supports every stage of your diagnostic workflow. We also specialize in sourcing rare-target raw materials to accelerate your project from concept to clinic.