Anti-GABARAPL2 Rabbit Monoclonal Antibody for WB, ELISA - P60520

Core Product Specifications and Parameters

Biological Background: GABARAPL2 Function and Localization

• GABARAPL2 (Gamma-aminobutyric acid receptor-associated protein-like 2) is a ubiquitin-like modifier also known as GATE-16, GEF-2, ATG8C, and MAP1 light chain 3-related protein.
• It functions in intra-Golgi traffic by modulating transport through coupling between NSF ATPase activity and SNARE activation.
• GABARAPL2 plays a critical role in autophagy and mitophagy, regulating mitochondrial quantity and quality; it is essential for a later stage in autophagosome maturation. Related references: PMID:20418806 PMID:23209295
• The protein localizes to cytoplasmic vesicles, autophagosomes, the endoplasmic reticulum membrane, and the Golgi apparatus.
• It is ubiquitously expressed, with high levels in brain, heart, prostate, ovary, spleen, and skeletal muscle, and low levels in lung, thymus, and small intestine.
• Post-translational modifications include acetylation and phosphorylation; the protein is a lipoprotein and membrane-associated, with a molecular weight of approximately 14 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 38-117, which may favor detection of linear epitopes; consider using this antibody in denaturing Western blot applications.
• For Western blotting, the predicted molecular weight of GABARAPL2 is about 14 kDa; use appropriate gel percentage (e.g., 12-15% SDS-PAGE) for good separation of low-molecular-weight proteins.
• The antibody is reported to cross-react with human, mouse, and rat samples; validate performance in your specific sample system using appropriate controls.
• For ELISA, optimize coating conditions and antibody dilutions empirically; consider indirect or sandwich ELISA formats depending on available reagents.
• This antibody may be suitable for detecting endogenous GABARAPL2 levels; if studying autophagy dynamics, note that the target is a ubiquitin-like modifier and may require lysis conditions that preserve lipidation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing specialized monoclonal antibodies like this anti-GABARAPL2 clone to support autophagy, Golgi transport, and neuroscience research. Beyond individual antibodies, we offer validated antibody pairs, optimized bulk reagents, and customized sourcing for rare targets, enabling seamless transition from assay development to clinical applications. Contact us to streamline your procurement and accelerate your diagnostic pipeline.