Anti-GABARAPL1 Rabbit Polyclonal Antibody for WB, ELISA - Q9H0R8

Core Product Specifications and Parameters

Biological Background: GABARAPL1 Function and Localization

• GABARAPL1 is a ubiquitin-like modifier protein belonging to the GABARAP/GATE-16 subfamily, distinct from LC3 family members, with roles in autophagy and receptor trafficking.
• It facilitates anterograde intracellular trafficking of kappa-type opioid receptor, increasing its cell-surface expression.
  Related references: PMID:16431922
• Essential for autophagosome maturation: while LC3s mediate phagophore elongation, GABARAPL1 functions at a later stage to complete autophagosome formation.
  Related references: PMID:20404487
• Participates in reticulophagy: through interaction with the receptor TEX264, it remodels ER subdomains into autophagosomes for ER turnover under nutrient stress.
  Related references: PMID:31006537 PMID:31006538
• Subcellularly, it localizes to autophagosomes, autophagosomal membranes, cytoplasm, cytoskeleton (microtubules), endoplasmic reticulum, and Golgi apparatus.
• Ubiquitously expressed but at very high levels in brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle; notably intense in brain motoneurons and substantia nigra pars compacta.
• The protein undergoes alternative splicing and is annotated with lipidation (lipoprotein), host-virus interaction, and reference proteome keywords.
• Its calculated molecular weight is ~14 kDa, consistent with the reported 14 kDa band.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (aa 1-50); this design may allow detection of full-length GABARAPL1 and potentially some splice variants.
• For Western blot, consider running whole-cell lysates from brain or heart tissues, where expression is high; expect a band near 14 kDa.
• ELISA can be performed using the immunizing peptide as a positive control; titrate the antibody to optimize signal-to-noise ratio.
• This antibody may be suitable for monitoring autophagy flux; paired with autophagy inhibitors (e.g., chloroquine), you may observe changes in GABARAPL1 lipidation or puncta formation.
• When probing for GABARAPL1 in immunofluorescence, note its association with autophagosomal and ER membranes; fixation and permeabilization conditions should be optimized.
• Cross-reactivity with mouse and rat is reported; verify reactivity in your specific sample system, as post-translational modifications or species differences may affect binding.

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CamelBio offers diagnostic manufacturers and research laboratories a comprehensive one-stop platform for IVD raw-material sourcing, including this GABARAPL1 rabbit pAb. Our portfolio spans validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, facilitating your workflow in autophagy and neuroscience research. Lean on our expertise to streamline procurement from concept to clinic.