Anti-GABARAPL1 Monoclonal Antibody for WB, IHC-P, ELISA - Q9H0R8

Core Product Specifications and Parameters

Biological Background: GABARAPL1 Function and Localization

• GABARAPL1 is a ubiquitin-like modifier that enhances cell-surface expression of kappa-type opioid receptor by promoting its anterograde trafficking. Related references: PMID:16431922
• It is involved in autophagosome formation; while LC3 proteins mediate phagophore elongation, GABARAP subfamily members are critical for later stages of autophagosome maturation. Related references: PMID:20404487
• Through its interaction with the reticulophagy receptor TEX264, GABARAPL1 mediates ER subdomain remodeling into autophagosomes during nutrient stress, facilitating lysosomal degradation for ER turnover. Related references: PMID:31006537 PMID:31006538
• The protein localizes to cytoplasmic vesicles, autophagosome membranes, the cytoskeleton, endoplasmic reticulum, and Golgi apparatus.
• It is ubiquitously expressed, with high levels in brain, heart, liver, kidney, placenta, and skeletal muscle, especially in motoneurons and the substantia nigra pars compacta.
• Alternative splicing produces multiple isoforms; the protein is a lipoprotein with a predicted molecular weight of ~14 kDa, but can be observed at ~17 kDa in tissues.
• Keywords include autophagy, host-virus interaction, proteomics identification, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1-100), which is expected to recognize all splicing isoforms.
• For Western blotting, the predicted molecular weight is ~14 kDa, but due to post-translational modifications, the observed band may be around 17 kDa. Use appropriate gel percentages and loading controls.
• For immunohistochemistry (IHC-P), consider antigen retrieval optimization and validate in known positive tissues (e.g., brain, heart) to confirm specificity.
• The antibody cross-reacts with mouse and rat orthologs, so it can be used in rodent models; however, always validate in the relevant sample system.
• For ELISA, a standard peptide/protein coating buffer and detection system should be applicable; titrate the antibody for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This anti-GABARAPL1 monoclonal antibody supports research in autophagy, neuroscience, and cellular trafficking, and CamelBio's integrated supply chain can efficiently meet your project needs at any development stage.