Anti-GABARAP Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95166

Core Product Specifications and Parameters

Biological Background: GABARAP Function and Localization

• Ubiquitin-like modifier involved in intracellular transport of GABA(A) receptors and interaction with the cytoskeleton. Related references: PMID:9892355
• Essential for a later stage in autophagosome maturation, distinct from the LC3 subfamily role in phagophore elongation. Related references: PMID:15169837 PMID:20562859 PMID:22948227
• Participates in reticulophagy by remodeling endoplasmic reticulum subdomains into autophagosomes upon nutrient stress, interacting with TEX264. Related references: PMID:31006538
• Required for local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating TIAM1 degradation and downstream signaling affecting cytoskeleton organization and cell migration. Related references: PMID:25684205
• Involved in apoptosis. Related references: PMID:15977068
• Mediates selective ER-phagy through interaction with UBAC2, promoting recruitment of UBAC2-associated ER fragments to autophagosomes. Related references: PMID:39284914
• Expressed in heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas.
• Subcellular localization includes autophagosome membrane, Golgi apparatus membrane, cytoplasmic vesicles, and the cytoskeleton.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 14 kDa; lipidated GABARAP-II may exhibit faster migration, so validate with positive and negative controls.
• In IHC-P, optimize antigen retrieval conditions and use tissues known to express GABARAP (e.g., brain, liver) as positive controls.
• For IF/ICC, fix and permeabilize cells under conditions that preserve organelle membranes to detect autophagosome-associated GABARAP.
• The N-terminal peptide immunogen (aa 1-100) may cross-react with related ATG8 family members (GABARAPL1, GABARAPL2); verify specificity if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips IVD manufacturers and research labs with a comprehensive range of raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-GABARAP polyclonal antibody supports autophagy, neuroscience, and cell death research, and our sourcing team can reliably supply rare-target raw materials to accelerate your assay development from concept to clinic.