Anti-GABARAP Polyclonal Antibody for WB, IHC-P, ELISA - O95166

Core Product Specifications and Parameters

Biological Background: GABARAP Function and Localization

• GABARAP (Gamma-aminobutyric acid receptor-associated protein), also known as MM46 or ATG8A, is a ubiquitin-like modifier belonging to the ATG8 family.
• It plays a critical role in the intracellular transport of GABA(A) receptors and their interaction with the cytoskeleton. Related references: PMID:9892355
• GABARAP is essential for a later stage in autophagosome maturation, distinguishing its function from LC3 proteins that act in phagophore elongation. Related references: PMID:15169837, PMID:20562859, PMID:22948227
• It is required for the local activation of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating TIAM1 degradation and downstream RAC1 signaling, thereby influencing cell migration and proliferation. Related references: PMID:25684205
• GABARAP participates in selective autophagy of the endoplasmic reticulum (reticulophagy/ER-phagy) through interactions with TEX264 and UBAC2, and has been implicated in apoptosis. Related references: PMID:31006538, PMID:15977068, PMID:39284914
• Subcellularly, GABARAP localizes to autophagosome membranes, cytoplasmic vesicles, the Golgi apparatus membrane, and the cytoskeleton.
• Tissue expression is broad, with high levels in heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas.
• GABARAP is a lipidated ubiquitin-like modifier, anchoring to autophagosomal membranes to execute its functions.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide corresponding to amino acids 1-100 of human GABARAP. For Western blot (WB), consider using cell or tissue lysates known to express GABARAP, such as brain or heart samples, with the predicted band at ~14 kDa. Validate in the relevant sample system to confirm specificity.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and validate in appropriate positive and negative control tissues, as GABARAP is broadly expressed.
• For ELISA, consider pairing with a capture antibody if used in sandwich format, and test with recombinant GABARAP protein or cell lysates.
• Given the high homology among GABARAP family members, cross-reactivity with GABARAPL1 or GABARAPL2 should be evaluated if specificity is critical.
• The antibody is provided as rabbit polyclonal; store at -20°C upon receipt and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, technical services, and consulting to support diagnostic manufacturers from concept to clinic. This anti-GABARAP antibody is part of our extensive catalog of validated antibodies for autophagy, neurology, and cancer research. For your procurement needs, we supply optimized antibody pairs, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to streamline your assay development.