Anti-GABARAP Monoclonal Antibody for WB, ELISA - O95166

Core Product Specifications and Parameters

Biological Background: GABARAP Function and Localization

• GABARAP (Gamma-aminobutyric acid receptor-associated protein, also known as MM46, ATG8A) is a ubiquitin-like modifier involved in intracellular transport and autophagy.
• It mediates the trafficking of GABA(A) receptors and their interaction with the cytoskeleton. Related references: PMID:9892355
• In autophagy, the GABARAP/GATE-16 subfamily is essential for the maturation stage of autophagosomes, distinguishing its role from the LC3 subfamily. Related references: PMID:15169837, PMID:20562859, PMID:22948227
• GABARAP participates in selective ER-phagy by interacting with reticulophagy receptors TEX264 and UBAC2 to facilitate endoplasmic reticulum turnover. Related references: PMID:31006538, PMID:39284914
• It is required for the activation of the CUL3(KBTBD6/7) ubiquitin ligase complex that targets TIAM1 for degradation, thereby regulating RAC1 signaling and downstream effects on cytoskeleton, cell migration, and proliferation. Related references: PMID:25684205
• GABARAP is also implicated in apoptosis. Related references: PMID:15977068
• It is localized to autophagosome and Golgi membranes, cytoplasmic vesicles, and the cytoskeleton, and is broadly expressed in tissues including brain, heart, liver, and kidney.

Experimental Guidance and Technical Tips

• The immunogen lies within the N-terminal 1-100 amino acid region of human GABARAP (O95166). For Western blot experiments, consider that the monomeric protein has a predicted molecular weight of 14 kDa, but post-translational lipidation may alter its migration pattern; validate the observed band in your sample system.
• When performing ELISA, use the immunogen peptide or recombinant GABARAP as a positive control to confirm antibody binding specificity.
• This antibody is stated to cross-react with mouse and rat; however, it is advisable to verify reactivity in your specific experimental system and tissue type before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports your autophagy and neuroscience research by offering validated antibody pairs, monoclonal and polyclonal antibodies like this GABARAP rabbit mAb, bulk ancillary reagents, and rare target sourcing. From concept to clinic, we streamline your procurement with expert technical support, ensuring you have the critical raw materials needed to advance diagnostic development and discovery.