Anti-GABA transaminase (ABAT) Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P80404

Core Product Specifications and Parameters

Biological Background: GABA Transaminase (ABAT) Function and Localization

• Encodes the mitochondrial enzyme 4-aminobutyrate aminotransferase (ABAT), also known as GABA aminotransferase (GABA-AT), which belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
• Catalyzes the conversion of gamma-aminobutyrate (GABA) to succinate semialdehyde and L-beta-aminoisobutyrate to methylmalonate semialdehyde, playing a central role in GABA degradation and neurotransmitter clearance. Related references: PMID:10407778 PMID:15528998
• Localizes specifically to the mitochondrial matrix, consistent with its transit peptide and role in mitochondrial metabolic pathways.
• Exhibits highest expression in liver, with additional expression in pancreas, brain, kidney, heart, and placenta.
• Utilizes pyridoxal phosphate as a cofactor and contains iron-sulfur clusters, highlighting its structural and catalytic requirements.
• Associated with disease variants linked to GABA-transaminase deficiency, emphasizing its clinical relevance in neurological and metabolic disorders.
• Keywords: Acetylation, Aminotransferase, Direct protein sequencing, Disulfide bond, Iron-sulfur, Metal-binding, Mitochondrion, Neurotransmitter degradation, Transferase.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein spanning amino acids 29–300 of human ABAT (NP_001120920.1), which may correspond to a portion of the mature mitochondrial protein after transit peptide cleavage. Users should validate specificity in their target sample system.
• Reported for use in WB, IF-P, and ELISA. For Western blot, consider a dilution range of 1:500–1:2000 with appropriate blocking and detection protocols.
• Cross-reactivity is demonstrated for human, mouse, and rat. When using mouse or rat lysates, verify a specific band at ~56 kDa, and account for potential species-specific isoforms.
• ABAT is a mitochondrial protein, so sample preparation may benefit from mitochondrial enrichment or lysis buffers optimized for organelle proteins to enhance detection sensitivity.
• For immunofluorescence on paraffin-embedded sections (IF-P), an antigen retrieval step (e.g., citrate buffer pH 6.0) may be necessary to unmask the epitope.
• ELISA-based applications may require optimization; pairing with a suitable capture antibody in a sandwich format is suggested if high-throughput detection is needed.

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