Anti-GABA A Receptor beta 2 (GABRB2) Rabbit Polyclonal Antibody for WB, ELISA - P47870

Core Product Specifications and Parameters

Biological Background: GABA A Receptor beta 2 (GABRB2) Function and Localization

• GABRB2 encodes the beta-2 subunit of ionotropic GABA(A) receptors, which function as heteropentameric ligand-gated chloride channels mediating fast inhibitory neurotransmission in the central nervous system (PubMed:19763268, PubMed:27789573, PubMed:29950725). Related references: PMID:19763268, PMID:27789573, PMID:29950725

• GABA(A) receptors are assembled from five subunits that form a central pore, with GABA binding sites located at the interface between alpha and beta subunits (PubMed:29950725). Related references: PMID:29950725

• Upon GABA binding, the channel opens, allowing chloride influx that hyperpolarizes the postsynaptic membrane and reduces neuronal excitability (By similarity).

• Certain subunit combinations, such as receptors containing alpha-1 and beta-2 or beta-3 subunits along with the gamma-2 subunit, exhibit synaptogenic activity, promoting rapid formation of synaptic contacts (PubMed:23909897, PubMed:25489750). Related references: PMID:23909897, PMID:25489750

• Extrasynaptic beta-2-containing receptors contribute to tonic GABAergic inhibition, regulating baseline neuronal activity.

• Beta subunit-containing GABA(A) receptors can simultaneously bind GABA and histamine at the interface of two neighboring beta subunits, a mechanism potentially involved in regulating sleep and wakefulness.

• Alternative splicing generates multiple isoforms; in disease, isoform 1 and 2 expression is reduced in schizophrenia, while isoform 3 is increased in schizophrenia and bipolar disorder.

• The protein is subject to post-translational modifications including glycosylation and phosphorylation, and is implicated in neurological disorders such as epilepsy; it localizes to the postsynaptic cell membrane, cell membrane, and cytoplasmic vesicle membrane.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal extracellular region (amino acids 26–244), which may detect both surface and internal pools of GABRB2; consider permeabilization protocols for intracellular detection.
• In Western blot, expect a primary band around 59 kDa; additional bands from isoforms or post-translational modifications may be observed.
• The antibody cross-reacts with mouse; validate in your specific mouse tissue or cell line to ensure specificity.
• For ELISA, the recombinant protein used as immunogen can serve as a positive control or standard curve reference.
• As a polyclonal antibody, it may recognize multiple epitopes; titrate carefully to minimize non-specific binding and confirm specificity using knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-GABRB2 polyclonal antibody supports neuroscience research on GABAergic signaling and related disorders, and CamelBio can assist with custom procurement of rare-target antibodies and one-stop technical support from concept to clinic. Whether you need optimized immunodetection reagents or tailored bulk supply solutions, our team is ready to bridge your sourcing requirements.