Anti-GAB1 Rabbit Polyclonal Antibody for WB and ELISA - Q13480

Core Product Specifications and Parameters

Biological Background: GAB1 Function and Localization

• GAB1 (GRB2-associated binding protein 1) is an adapter protein that plays a core role in intracellular signaling cascades downstream of activated receptor tyrosine kinases, including FGFR1, EGFR, and INSR.
• Alternative splicing of the GAB1 gene produces multiple isoforms, which may contribute to functional diversity in different cellular contexts.
• GAB1 is critically involved in the MET/HGF signaling pathway, mediating processes such as cell growth, migration, and morphogenesis. Related references: PMID:29408807
• Genetic variants in GAB1 are associated with non-syndromic deafness (DFNB26), highlighting its importance in auditory function.
• Post-translational modifications, including phosphorylation and acetylation, regulate GAB1 activity, localization, and interaction with downstream effectors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 594-694) of human GAB1. For Western blot, a band around 77 kDa representing full-length GAB1 is expected; additional isoforms or cleavage products may also be detected.
• This antibody has been validated for WB and ELISA applications. For WB, use appropriate protein loading controls and optimize antibody dilution for your specific sample type.
• Cross-reactivity is reported for human, mouse, and rat samples. When testing other species, sequence homology should be examined, and validation in the relevant sample system is recommended.
• For ELISA, consider pairing this antibody with a capture antibody targeting a non-overlapping epitope to ensure robust detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic manufacturers and research labs with high-quality reagents from concept to clinic. For GAB1-related research in cell signaling and hearing loss, we offer validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents to support your assay development. Our one-stop platform ensures efficient procurement of rare target raw materials, paired with expert technical support to streamline your workflow.