Anti-G6PD Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P11413

Core Product Specifications and Parameters

Biological Background: G6PD Function and Localization

• G6PD is glucose-6-phosphate 1-dehydrogenase, the rate-limiting enzyme of the oxidative pentose phosphate pathway. It produces NADPH and pentose phosphates essential for reductive biosynthesis and nucleic acid synthesis.
• Alternative splicing generates multiple isoforms; isoform Long is predominantly expressed in lymphoblasts, granulocytes, and sperm.
• G6PD is primarily cytosolic but can associate with cellular membranes.
• In addition to its metabolic role, G6PD catalyzes the conversion of NAADPH back to NAADP, thereby regulating Ca²⁺ signaling during T cell activation. Related references: PMID:34784249
• Post-translational modifications include acetylation, phosphorylation, and hydroxylation.
• Mutations in G6PD cause glucose-6-phosphate dehydrogenase deficiency, a common enzymopathy leading to hemolytic anemia.
• As an oxidoreductase, G6PD uses NADP as a cofactor and plays a central role in maintaining cellular redox balance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 415–515 of human G6PD (isoform NP_001035810.1), a region near the C-terminus; consider its accessibility and conservation across species when designing experiments.
• For Western blotting using polyclonal antibodies, start with a dilution of 1:500–1:2000 and optimize based on signal-to-noise ratio in your sample.
• In immunofluorescence (IF/ICC), validate fixation and permeabilization protocols; paraffin-embedded sections (IF-P) may require antigen retrieval.
• ELISA applications may benefit from pairing this antibody with a matched detection antibody; consider using recombinant protein standards for quantitation.
• Since this is a polyclonal antibody, batch-to-batch variability may occur; include appropriate controls and consider re-validation for each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For G6PD-related assay development, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you need routine raw materials or hard-to-find targets, our supply chain expertise ensures reliable access to the critical components for your diagnostic pipelines.