Anti-G6PD Rabbit Monoclonal Antibody for WB, ELISA - P11413

Core Product Specifications and Parameters

Biological Background: G6PD Function and Localization

• G6PD (Glucose-6-phosphate 1-dehydrogenase) is a cytosolic enzyme encoded by the G6PD gene; isoform Long is specifically expressed in lymphoblasts, granulocytes, and sperm.
• It catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, providing NADPH and pentose phosphates essential for fatty acid and nucleic acid synthesis.
• Additionally, G6PD mediates the conversion of NAADPH back to NAADP, promoting Ca2+ signaling during T cell activation, as revealed by recent studies. Related references: PMID:34784249
• The enzyme localizes to the cytoplasm, cytosol, and membrane, consistent with its roles in cellular redox balance and metabolic signaling.
• Post-translational modifications include acetylation and phosphorylation; it is associated with disease variants linked to hereditary hemolytic anemia and glucose-6-phosphate dehydrogenase deficiency.
• Keywords: Oxidoreductase, NADP, carbohydrate metabolism, glucose metabolism, alternative splicing, and reference proteome.

Experimental Guidance and Technical Tips

• For Western blot (WB), use denaturing and reducing conditions; expect a band at approximately 59 kDa. Validate detection in your specific sample type.
• For ELISA applications, consider using the immunogenic peptide (aa 400-500) as a coating antigen; optimize coating concentration and blocking conditions.
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency; include appropriate positive and negative controls in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a reliable supply of IVD raw materials, including this high-quality G6PD monoclonal antibody for metabolism and anemia-related studies. Our portfolio features validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your projects from concept to clinical application.