Anti-G6PD Polyclonal Antibody for WB, IF/ICC, ELISA - P11413

Core Product Specifications and Parameters

Biological Background: G6PD Function and Localization

• Glucose-6-phosphate 1-dehydrogenase, encoded by the G6PD gene; isoform Long is expressed in lymphoblasts, granulocytes, and sperm.
• Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, generating NADPH and pentose phosphates essential for fatty acid and nucleic acid synthesis.
• Converts NAADPH (produced by DUOX1, DUOX2, and NOX5 from NAADP) back to NAADP, thereby promoting Ca(2+) signaling during T cell activation. Related references: PMID:34784249
• Subcellular localization includes cytoplasm, cytosol, and membrane.
• Mutations in G6PD are associated with hereditary hemolytic anemia, classified as a disease variant.
• Post-translational modifications include acetylation, phosphorylation, and hydroxylation; alternative splicing yields multiple isoforms.
• Keywords highlight its roles in carbohydrate metabolism, glucose metabolism, NADP binding, and oxidoreductase activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 195–515, covering a large portion of the protein and likely providing robust antigenicity; consider pre-adsorption if cross-reactivity is observed.
• For Western blot, a band near 59 kDa is expected; validate in the appropriate sample system (e.g., human, mouse, or rat lysates) with proper loading controls.
• In IF/ICC experiments, cytoplasmic and potential membrane staining is anticipated; optimize fixation and permeabilization conditions for your cell type.
• For ELISA, this polyclonal antibody can serve as a capture or detection reagent; pair with a matched monoclonal antibody for sandwich assays and validate with known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with validated antibodies and ancillary reagents. This anti-G6PD polyclonal antibody supports enzymopathy and metabolic disorder research, including pentose phosphate pathway studies and G6PD deficiency investigations. We offer a broad portfolio of ready-to-use antibody pairs, bulk monoclonal/polyclonal antibodies, and custom sourcing for rare targets to streamline your diagnostic development from concept to clinic.