Anti-G6PC Rabbit Polyclonal Antibody for WB, ELISA - P35575

Core Product Specifications and Parameters

Biological Background: G6PC Function and Localization

• G6PC (Glucose-6-phosphatase catalytic subunit 1) is an endoplasmic reticulum membrane protein that catalyzes the hydrolysis of glucose-6-phosphate to glucose.
• It functions in a complex with the glucose-6-phosphate transporter (SLC37A4/G6PT) to mediate the terminal step of glycogenolysis and gluconeogenesis.
• G6PC is a key enzyme in homeostatic regulation of blood glucose levels.
• The protein undergoes alternative splicing, generating multiple isoforms.
• Subcellular localization is the endoplasmic reticulum membrane, with multiple transmembrane helices.
• Mutations in G6PC are associated with glycogen storage disease type Ia (GSD1a).
• G6PC is a glycoprotein, and its 3D structure has been determined.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200–300; confirm target sequence alignment across species for cross-reactivity.
• For Western blot (WB) under denaturing conditions, expect a band near 40 kDa; note that glycosylation or other modifications may affect apparent molecular weight.
• For ELISA applications, the antibody may be used as a capture or detection reagent; optimize coating and blocking conditions experimentally.
• Validate antibody performance in biologically relevant samples, such as liver or kidney tissues, which express high levels of G6PC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, including optimized antibodies for glucose metabolism targets like G6PC. Whether you require validated antibody pairs, bulk monoclonal/polyclonal reagents, or supplementary detection components, our one-stop platform supports every stage of assay development from concept to clinic.