Anti-G3BP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UN86

Core Product Specifications and Parameters

Biological Background: G3BP2 Function and Localization

• G3BP2 (Ras GTPase-activating protein-binding protein 2), also known as GAP SH3 domain-binding protein 2, is a 54kDa scaffold protein with key roles in cytoplasmic stress granule dynamics.
• Plays an essential role in cytoplasmic stress granule formation: these membraneless compartments store mRNAs and stalled translation pre-initiation complexes in response to stress (PubMed:27022092, PubMed:32302570, PubMed:32302571, PubMed:32302572). Related references: PMID:27022092, PMID:32302570, PMID:32302571, PMID:32302572
• Promotes liquid-liquid phase separation (LLPS) of stress granules upon binding unfolded RNA, acting as a molecular switch triggered by increased intracellular free RNA concentrations (By similarity).
• Functions as a platform for antiviral signaling within stress granules, contributing to innate immunity (PubMed:23279204, PubMed:32302570, PubMed:32302571). Related references: PMID:23279204, PMID:32302570, PMID:32302571
• Subcellular localization: predominantly cytoplasmic, and accumulates in stress granules upon stress induction.
• Post-translational modifications include phosphorylation, methylation, and ubiquitin-like conjugation; associated keywords include mRNA transport, host-virus interaction, and isopeptide bond.
• UniProt molecular weight is 54121 Da, consistent with the observed 54kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 140-280 of human G3BP2, a region likely conserved in mouse and rat, supporting observed cross-reactivity.
• For Western blot (WB), expect a band near 54kDa; validate using lysates from relevant cell lines or tissues expressing G3BP2.
• When studying stress granules, consider inducing cellular stress (e.g., arsenite treatment) to enhance G3BP2 puncta formation and detection.
• For immunohistochemistry (IHC-P), antigen retrieval and blocking steps may require optimization; inclusion of positive and negative controls is advised.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a matched antibody pair might improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD manufacturers and research laboratories, offering validated raw materials such as anti-G3BP2 antibodies for stress granule and antiviral signaling research. We supply polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents in bulk, with technical support to accelerate assay development. For rare targets or customized requirements, CamelBio provides tailored sourcing solutions to bridge your concept-to-clinic workflow.