Anti-G3BP1 Rabbit Monoclonal Antibody [KO Validated] for WB, IF/ICC, IHC-P, ELISA - Q13283

Core Product Specifications and Parameters

Biological Background: G3BP1 Function and Localization

• G3BP1 is a multifunctional protein essential for stress granule formation through liquid-liquid phase separation (LLPS), storing mRNAs and stalled translation complexes under stress. Related references: PMID:37672657 PMID:20180778 PMID:23279204 PMID:32302570 PMID:32302571 PMID:32302572
• It functions as an ATP- and magnesium-dependent DNA/RNA helicase, unwinding various nucleic acid duplexes with 5' to 3' directionality. Related references: PMID:9889278
• Plays a critical role in innate immunity by promoting CGAS/STING pathway activation and RIGI-mediated interferon production, and inducing cGAS condensation. Related references: PMID:30510222 PMID:30804210 PMID:34779554
• May act as a phosphorylation-dependent sequence-specific endoribonuclease, cleaving between C and A, with preference for MYC mRNA 3'-UTR. Related references: PMID:11604510
• Localizes to the cytoplasm, cytosol, perikaryon, stress granules, and nucleus, supporting its diverse functional roles.
• Ubiquitously expressed across tissues, with documented involvement in host-virus interactions.
• Subject to multiple post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitination, modulating its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 210-323) targets a central region of G3BP1, so the antibody may detect both the full-length 52 kDa protein and a smaller 14 kDa isoform or fragment. In Western blot, consider loading adequate protein and using appropriate positive controls to confirm band specificity.
• For immunofluorescence (IF/ICC), stress granule induction (e.g., by arsenite treatment) may be required to observe robust punctate staining; validate in your cell system with and without induction.
• This antibody is validated for knockout (KO) applications; for quantitative studies, consider using KO cell lines as negative controls to ensure specificity.
• For IHC-P, antigen retrieval methods may be necessary; optimize per tissue type and fixation conditions.
• Cross-reactivity with mouse and rat G3BP1 has been reported, but confirm sequence homology in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing IVD raw materials and technical support to diagnostic manufacturers and research laboratories, offering a seamless path from concept to clinic. This anti-G3BP1 rabbit monoclonal antibody exemplifies our commitment to supplying validated tools for stress granule biology and innate immunity research. Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and customized sourcing for rare or emerging targets to accelerate your assay development.