Anti-G3BP1 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q13283

Core Product Specifications and Parameters

Biological Background: G3BP1 Function and Localization

• Ras GTPase-activating protein-binding protein 1 (G3BP1), also designated ATP-dependent DNA helicase VIII (hDH VIII) and GAP SH3 domain-binding protein 1, is a multifunctional protein involved in stress granule dynamics, RNA metabolism, and innate immunity.
• G3BP1 is a critical nucleator of stress granules, membraneless organelles that sequester mRNAs and stalled translation complexes under stress. It drives liquid-liquid phase separation (LLPS) upon binding unfolded RNA, serving as a molecular switch that senses free RNA levels to trigger condensation.
  Related references:
  PMID:37672657 PMID:20180778 PMID:23279204 PMID:32302570 PMID:32302571 PMID:32302572
• Exhibits ATP- and magnesium-dependent helicase activity, unwinding DNA/DNA, RNA/DNA, and RNA/RNA duplexes with a 5'→3' directionality, preferring partial duplexes with a 17-bp annealed region and 3' overhangs.
  Related references:
  PMID:9889278
• Enhances innate immune signaling by promoting the DNA-sensing cGAS-STING pathway and the RNA-sensing RIG-I pathway. It facilitates DNA binding and condensation of cGAS, and boosts RIG-I-mediated type I interferon production.
  Related references:
  PMID:30510222 PMID:30804210 PMID:34779554
• Possesses phosphorylation-dependent endoribonuclease activity in vitro, cleaving between cytosine and adenine with a preference for the 3'-UTR of MYC mRNA.
  Related references:
  PMID:11604510
• Localizes to the cytoplasm, cytosol, perikaryon, nucleus, and prominently to stress granules upon cellular insult.
• Ubiquitously expressed across tissues and subject to multiple post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its function and localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350-450 of human G3BP1 (Q13283), a region within the C-terminal domain. This sequence is highly conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blot (WB), a specific band at approximately 52 kDa is expected. Optimize blocking and antibody dilution for your sample type.
• In immunofluorescence (IF/ICC), G3BP1 displays diffuse cytoplasmic staining under basal conditions; stress granule induction (e.g., by arsenite treatment) may be required to observe distinct punctate localization.
• For immunoprecipitation (IP), confirm capture efficiency under your lysis and wash conditions, as G3BP1 interactions with RNA and other proteins may influence recovery.
• For ELISA, the antibody can be used as a capture or detection reagent; validate pairing with a compatible secondary antibody or antigen.
• Always validate antibody performance in the relevant sample system and application, as experimental conditions can affect results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering a comprehensive portfolio of monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents. For researchers investigating stress granule biology, mRNA regulation, and innate immunity, we provide streamlined access to critical reagents like anti-G3BP1 antibodies, accelerating your project from concept to clinical application. Our one-stop sourcing model simplifies supply chain management, ensuring consistent quality and technical support for diagnostic developers and laboratories.