Anti-G3BP1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13283

Core Product Specifications and Parameters

Biological Background: G3BP1 Function and Localization

• G3BP1 (Ras GTPase-activating protein-binding protein 1), also known as GAP SH3 domain-binding protein 1 (HDH-VIII), is a multifunctional protein involved in stress granule formation, innate immunity, and nucleic acid unwinding.
• Plays an essential role in stress granule formation by promoting the formation of membraneless organelles through RNA-binding-induced liquid-liquid phase separation (LLPS). Stress granules store translationally stalled mRNAs and proteins under stress conditions. Related references: PMID:37672657, PMID:20180778, PMID:23279204
• Functions as an ATP- and magnesium-dependent DNA/RNA helicase (hDH VIII) that unwinds DNA/DNA, RNA/DNA, and RNA/RNA duplexes with 5'→3' directionality, preferentially on substrates with 3' overhangs. Related references: PMID:9889278
• Participates in innate immune signaling: promotes the DNA binding and condensation of cGAS to activate the cGAS/STING pathway, and enhances RIG-I-mediated type I interferon production upon sensing pathogenic RNA. Related references: PMID:30510222, PMID:30804210
• Also reported to act as a phosphorylation-dependent sequence-specific endoribonuclease that cleaves between cytosine and adenine, and MYC mRNA at the 3'-UTR in vitro. Related references: PMID:11604510
• Ubiquitous tissue expression; subcellularly localized mainly in the cytoplasm, cytosol, perikaryon, and nucleus, and relocalizes to stress granules upon stress.
• Post-translationally modified by acetylation, methylation, phosphorylation, and ubiquitination, reflecting dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen spans a central region (aa 131-330) of human G3BP1, conserved across human, mouse, and rat, which likely contributes to the observed cross-reactivity.
• For Western blot (WB), an expected band around 52 kDa should be detected; consider appropriate positive/negative controls and optimize primary antibody dilution.
• In immunofluorescence/immunocytochemistry (IF/ICC), stress induction (e.g., arsenite treatment) may be necessary to visualize stress granule localization; verify permeabilization and blocking steps.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and antibody titration are critical; staining patterns may vary by tissue type.
• ELISA performance may require optimization of coating antigen concentration and detection system for reliable quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your G3BP1 research and assay development with a comprehensive portfolio of IVD raw materials. From this high-quality rabbit polyclonal antibody to validated antibody pairs and bulk ancillary reagents, we streamline sourcing for diagnostic manufacturers and labs. Our commitment to one-stop service bridges the gap between concept and clinic, empowering your innovations in stress granule biology and innate immunity.