Anti-G2E3 Rabbit Polyclonal Antibody for WB and ELISA - Q7L622

Core Product Specifications and Parameters

Biological Background: G2E3 Function and Localization

• G2E3, also known as PHF7B or KIAA1333, is a G2/M phase-specific HECT-type E3 ubiquitin-protein ligase.
• It catalyzes the monoubiquitination of histone H3 at Lys-14 (H3K14ub), a mark essential for heterochromatin formation at pericentric regions. Related references: PMID:41094145
• H3K14ub facilitates the deposition of H3K9me3 and subsequent recruitment of SUV39H and HP1 proteins, thereby establishing constitutive heterochromatin. Related references: PMID:41094145
• G2E3 associates with mitotic chromosomes in an RNA-dependent manner to perform its ubiquitination function.
• Subcellularly, it localizes to the nucleus, chromosomes, and nucleolus, with some cytoplasmic presence.
• Predominantly expressed in brain, liver, kidney, testes, and ovary.
• It features zinc-finger domains and a HECT domain, classifying it as a transferase involved in ubiquitin conjugation.

Experimental Guidance and Technical Tips

• For WB, consider testing with whole cell lysates from human, mouse, or rat samples, and validate detection of the ~81 kDa band.
• ELISA may be used to detect G2E3 in recombinant or native samples; ensure appropriate antigen coating.
• The immunogen covers residues 1-180; confirm specificity using positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-G2E3 polyclonal antibody supports cell cycle and chromatin research. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline your assay development.