Anti-G0S2 Polyclonal Antibody for WB, IHC-P, ELISA - P27469

Core Product Specifications and Parameters

Biological Background: G0S2 Function and Localization

• G0S2 (G0/G1 switch protein 2), also known as G0/G1 switch regulatory protein 2, is a small protein of approximately 11 kDa encoded by the G0S2 gene in humans.
• It functions as a pro-apoptotic factor by directly binding to BCL2, thereby preventing the formation of anti-apoptotic BCL2-BAX heterodimers.
• Localizes primarily to the mitochondria, supporting its role in the intrinsic apoptosis pathway.
• Widely expressed across tissues, with highest levels in peripheral blood, skeletal muscle, and heart, and moderate expression in kidney and liver.
• Contains domains involved in protein-protein interactions with BCL2 family members, critical for its regulatory function in apoptosis.
• Post-translational modifications and structural details remain under investigation; proteomics studies confirm its identification in human samples.
• Belongs to the G0S2 family and is conserved across species (human, mouse, rat as supported by cross-reactivity data).

Experimental Guidance and Technical Tips

• Immunogen design: The immunogen used is a synthetic peptide spanning amino acids 1–100 of human G0S2 (NP_056529.1). This N-terminal region may elicit antibodies recognizing the native protein; consider validating specificity in the relevant sample system.
• Recommended applications: This antibody is suitable for WB, IHC-P, and ELISA. For WB, use a loading control appropriate for ~11 kDa. In IHC-P, expect mitochondrial staining; optimize antigen retrieval and blocking conditions.
• Cross-reactivity: Predicted to react with human, mouse, and rat based on sequence homology. For non-human samples, perform preliminary sequence alignment and validation experiments.
• Technical note: As a polyclonal antibody, verify batch-to-batch consistency for critical assays. Include negative controls such as G0S2-low or knockout cell lines/tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, specializing in apoptosis and mitochondrial research targets like G0S2. Our supply capabilities include validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development. For rare or custom targets, we bridge the gap from concept to clinic, ensuring you have the right raw materials at every stage.