Anti-FZD9 Rabbit Polyclonal Antibody for WB and ELISA - O00144

Core Product Specifications and Parameters

Biological Background: Frizzled-9 Function and Localization

• Frizzled-9 functions as a receptor for WNT2, initiating the beta-catenin canonical signaling pathway that leads to disheveled activation, GSK-3 inhibition, and nuclear accumulation of beta-catenin, thereby activating Wnt target genes (By similarity).
• In the neuromuscular junction (NMJ), Frizzled-9 negatively regulates the clustering of acetylcholine receptors (AChR) through the beta-catenin canonical signaling pathway, contributing to proper NMJ assembly (By similarity).
• Frizzled-9 supports neural progenitor cell (NPC) viability by inhibiting cell cycle arrest and neuronal apoptosis via the beta-catenin pathway (PubMed:27509850). Related references: PMID:27509850
• During hippocampal development, Frizzled-9 regulates neuroblast proliferation and apoptotic cell death, influencing brain architecture formation.
• Frizzled-9 controls bone formation and positively regulates bone regeneration through non-canonical Wnt signaling mediated by ISG15 (By similarity).
• The protein is localized to the cell membrane and contains characteristic features of a G protein-coupled receptor (GPCR), including a signal peptide, seven transmembrane domains, disulfide bonds, and glycosylation sites.
• Frizzled-9 is predominantly expressed in adult and fetal brain, testis, eye, skeletal muscle, and kidney, with moderate expression in pancreas, thyroid, adrenal cortex, small intestine, and stomach; it is also detected in fetal liver and kidney.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular region (aa 469-591) of human FZD9, suggesting that the antibody recognizes an intracellular epitope. For Western blotting, use reducing and denaturing conditions to fully expose the epitope.
• Predicted molecular weight is ~64 kDa; however, glycosylation may result in a higher observed band. Validate the band pattern in relevant tissue or cell lysates (e.g., brain, testis, kidney) alongside appropriate controls.
• This rabbit polyclonal antibody is suitable for WB and ELISA applications. For WB, a starting dilution of 1:500–1:2000 is often effective; optimize based on your experimental system. For ELISA, perform checkerboard titrations to determine the optimal coating antigen concentration and antibody dilution.
• Cross-reactivity with mouse and rat FZD9 enables comparative studies across species; confirm sequence homology in the immunogen region to anticipate detection.
• As a polyclonal antibody, it may detect multiple isoforms or degradation products. Include a loading control and, if necessary, validate specificity with knockout or knockdown samples.
• Shipping: provided on ice bags to maintain stability; store at -20°C upon receipt and avoid repeated freeze-thaw cycles.

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CamelBio streamlines IVD raw material procurement, offering one-stop access to validated antibodies, recombinant proteins, and ancillary reagents for Wnt signaling and neurodevelopment research. This FZD9 rabbit polyclonal antibody is part of our extensive catalog, supported by bulk supply options and customized sourcing for rare targets. Let CamelBio be your trusted partner from concept to clinic.