Anti-FZD7 Rabbit Polyclonal Antibody for WB, ELISA - O75084

Core Product Specifications and Parameters

Biological Background: Frizzled-7 Function and Localization

• Frizzled-7 (FZD7) is a receptor for Wnt proteins, primarily activating the beta-catenin canonical signaling pathway.
• It leads to inhibition of GSK-3 kinase and nuclear accumulation of beta-catenin, ultimately activating Wnt target genes.
• Some FZD7-mediated signaling involves PKC and calcium fluxes, potentially integrating with the canonical pathway.
• Upon ligand activation, FZD7 binds to CCDC88C/DAPLE, displacing DVL1 and inhibiting canonical Wnt signaling while triggering non-canonical responses via G-protein activation. Related references: PMID:26126266
• Acts as a cell surface receptor for Clostridium difficile toxin TcdB in colonic epithelium during microbial infection.
• Localizes to the cell membrane and endosome membrane.
• Expressed at high levels in adult skeletal muscle, fetal kidney, fetal lung, adult heart, brain, and placenta; notably expressed in squamous cell esophageal carcinomas.
• Belongs to the G protein-coupled receptor family and contains disulfide bonds and glycosylation sites; involved in development and Wnt signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-260 of human FZD7, covering an extracellular region; this design may be suitable for detecting cell surface expression.
• For Western blot, a band around 64 kDa is expected under reducing conditions; validate the antibody in your specific sample system.
• For ELISA, consider using a rabbit IgG-specific detection conjugate and optimize coating conditions with the recombinant immunogen or cell lysates.
• When working with mouse samples, verify cross-reactivity and signal specificity, as reactivity has been predicted but not experimentally confirmed for all assays.
• Store at recommended temperatures and avoid repeated freeze-thaw cycles to maintain antibody performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This FZD7 polyclonal antibody supports Wnt signaling research and assay development in human and mouse systems. Explore our portfolio for validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic programs.