Anti-FZD6 Polyclonal Antibody for WB, ELISA - O60353

Core Product Specifications and Parameters

Biological Background: FZD6 Function and Localization

• Frizzled-6 (FZD6) is a receptor for Wnt proteins, coupling primarily to the beta-catenin canonical signaling pathway and potentially involving PKC and calcium flux.
• It plays a role in transduction and intercellular transmission of polarity information during tissue morphogenesis and planar cell polarity (PCP) establishment.
• Together with FZD3, FZD6 is involved in neural tube closure and the orientation of asymmetric stereocilia bundles in inner ear sensory cells (By similarity).
• Subcellularly, FZD6 localizes to the cell membrane, apical cell membrane, cytoplasmic vesicle membrane, and endoplasmic reticulum membrane.
• The protein is widely expressed in adult tissues (heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine, colon) and fetal tissues (brain, lung, liver, kidney).
• FZD6 is a member of the G-protein coupled receptor Frizzled family, featuring a signal peptide, seven transmembrane domains, disulfide bonds, and sites for phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 19-201), suggesting the antibody may recognize multiple FZD6 isoforms or processed forms; validate in relevant sample systems.
• For Western blot, a band of approximately 79 kDa is expected; consider using positive control lysates from tissues such as brain or kidney, which show high FZD6 expression.
• The antibody cross-reacts with mouse and rat FZD6, enabling comparative studies across species; ensure appropriate species-specific controls.
• For ELISA, the recombinant immunogen can serve as a positive control for assay development and validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials and technical support. For investigators studying Wnt signaling, planar cell polarity, or developmental biology, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies against FZD6, and bulk ancillary reagents. Our one-stop sourcing model bridges gaps from concept to clinic, supporting your entire workflow.