Anti-FZD4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9ULV1

Core Product Specifications and Parameters

Biological Background: Frizzled-4 Function and Localization

• Frizzled-4 (FZD4) is a receptor for Wnt proteins, coupling to the beta-catenin canonical signaling pathway, leading to activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin, and activation of Wnt target genes (PubMed:30135577). Related references: PMID:30135577
• FZD4 plays a critical role in retinal vascularization by acting as a receptor for both Wnt proteins and norrin (NDP), promoting beta-catenin accumulation and LEF/TCF-mediated transcription.
• A second signaling pathway involving PKC and calcium fluxes has been observed for some frizzled family members, but its relationship to the canonical pathway and dependence on G proteins is not yet fully elucidated.
• Frizzled-4 is a multi-pass transmembrane receptor localized to the cell membrane, featuring a signal peptide and a cysteine-rich extracellular domain critical for ligand binding.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitin conjugation, contributing to its structural and functional regulation.
• FZD4 is almost ubiquitously expressed, with highest levels in adult heart, skeletal muscle, ovary, and fetal kidney, and moderate amounts in liver, kidney, pancreas, spleen, and fetal lung (PubMed:10544037). Related references: PMID:10544037
• Belongs to the G-protein coupled receptor Fz/Smo family and participates in developmental processes, tissue morphogenesis, and intercellular transmission of polarity information.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 42-179) targets the extracellular N-terminal region, which may allow detection of both native and denatured forms; consider optimizing conditions for each application individually.
• For WB, a major band around 60kDa is expected; be aware that post-translational modifications could cause slight shifts in migration.
• In IHC-P, antigen retrieval steps should be optimized for the extracellular epitope; staining is anticipated at the cell membrane.
• Cross-reactivity to mouse and rat is indicated, making it suitable for translational studies, but verify with appropriate species-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

This FZD4 polyclonal antibody supports research into Wnt signaling and retinal vascularization, critical for developmental biology and disease studies. CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your diagnostic development from concept to clinic.