Anti-FYB Polyclonal Antibody for WB, ELISA - O15117

Core Product Specifications and Parameters

Biological Background: FYN-binding protein 1 (FYB) Function and Localization

• FYB functions as an adapter protein in T-cells, linking FYN and LCP2 signaling cascades to actin cytoskeleton remodeling (PubMed:10747096, PubMed:16980616).
  Related references:
  PMID:10747096
  PMID:16980616

• FYB prevents the proteasomal degradation of SKAP1 and SKAP2, scaffolding proteins critical for immune cell signaling (PubMed:15849195).
  Related references:
  PMID:15849195

• The protein modulates IL2 expression and participates in platelet activation and mast cell high affinity IgE receptor signaling.

• FYB is encoded by the FYB1 gene and undergoes alternative splicing to produce multiple isoforms, including p120 and p130.

• It localizes to the cytoplasm, nucleus, and cell junctions, facilitating signal transduction and cytoskeletal reorganization.

• Tissue expression is predominantly in hematopoietic tissues such as myeloid cells, T-cells, spleen, and thymus, with absent expression in B-cells and non-lymphoid tissues.

• The protein is subject to acetylation and phosphorylation, and contains an SH3 domain and coiled-coil repeats, enabling diverse protein interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment encompassing the C-terminal region (aa 678-829) of human FYB. This region may confer cross-reactivity to mouse FYB, as suggested by the reported species reactivity; consider sequence alignment to verify conservation.
• For Western blotting, the predicted molecular weight is approximately 85 kDa, but alternative splicing may yield additional bands. Optimize antibody dilution and validate against relevant cell lysates (e.g., Jurkat T-cells).
• ELISA applications can utilize recombinant FYB protein or peptide to assess binding specificity; ensure proper coating and use appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic developers focusing on immune signaling adaptors like FYB, we offer this rabbit polyclonal antibody as part of our comprehensive portfolio, complemented by technical support to help streamline your development from concept to clinic. Whether you need rare target reagents or large-scale manufacturing supplies, CamelBio bridges the gap between innovative research and clinical application.