Anti-FXYD6 Polyclonal Antibody for WB, ELISA - Q9H0Q3

Core Product Specifications and Parameters

Biological Background: FXYD6 Function and Localization

• FXYD6 encodes FXYD domain-containing ion transport regulator 6, also known as phosphohippolin, a single-span transmembrane protein of the FXYD family.
• It associates with and modulates the activity of the Na+/K+-ATPase (sodium pump), reducing the apparent affinity for intracellular Na+ without affecting extracellular K+ affinity (PubMed:33231612). Related references: PMID:33231612
• FXYD6 may also act as a chaperone to regulate the proper localization and trafficking of the Na+/K+-ATPase to the plasma membrane (By similarity).
• The protein localizes primarily to the cell membrane, consistent with a transmembrane regulatory role.
• It contains a signal peptide and one predicted transmembrane helix, with reported alternative splicing contributing to potential functional diversity.
• Key functional annotations include sodium ion transport, potassium ion transport, and ion homeostasis, highlighting its role in maintaining cellular electrochemical gradients.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length extracellular domain (aa 1-95), which may help target the native conformation in membrane fractions; consider validating detection in whole-cell lysates versus membrane-enriched preparations.
• Predicted molecular weight is approximately 11 kDa; a 12–15% SDS-PAGE gel is recommended for optimal separation in western blotting.
• Cross-reactivity with mouse and rat is indicated, making this antibody useful for translational models; however, verify expression levels and band patterns in relevant tissue or cell types.
• For ELISA, ensure the recombinant protein or synthetic peptide coating matches the immunogen sequence to assess antibody specificity and titer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and laboratories with streamlined access to a broad portfolio of IVD-critical raw materials, including antibodies for ion transport and membrane protein targets such as FXYD6. Our one-stop platform supports your entire development workflow—from antibody selection and bulk ordering to technical consultation and custom reagent sourcing—helping you accelerate assay development from concept to clinical application.